LOCUS       40924_25561       12929 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pInSRT-GFPV1 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12929)
  AUTHORS   Wasa J, Nishida Y, Shinomura T, Urakawa H, Arai E, Futamura N, 
            Tsukushi S, Ishiguro N.
  TITLE     Versican Regulates Cell-Associated Matrix Formation and Cell 
            Behavior Differentially from Aggrecan in Swarm Rat Chondrosarcoma 
            Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12929)
  AUTHORS   Shinomura T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2010) Contact:Tamayuki Shinomura Tokyo Medical and
            Dental University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-Ku, 
            Tokyo 113-8549, Japan
REFERENCE   3  (bases 1 to 12929)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12929)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-JUN-2010) Contact:Tamayuki Shinomura Tokyo Medical and Dental 
            University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-Ku, Tokyo 
            113-8549, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        14..32
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    50..97
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    177..236
                     /gene="GFPV1"
                     /label=signal sequence derived from human PG-M/versican
                     /note="signal sequence derived from human PG-M/versican"
     CDS             279..995
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    8297..8883
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             8884..9480
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    9570..9794
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(9828..9846)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9853..9869)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(10010..10465)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        10799..10903
                     /label=AmpR promoter
     CDS             10904..11761
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11935..12523
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    12811..12832
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        12847..12877
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12885..12901
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12909..12925
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 attacgccaa gctatttagg tgacactata gaatactcaa gcttcgaccg aagttcctat
       61 tccgaagttc ctattctcta gaaagtatag gaacttcgga tcccctaatt caagcttgcg
      121 cagcgctgca gtgaattttc cccccaaact gcaataagcc gccttccaag gccaagatgt
      181 tcataaatat aaagagcatc ttatggatgt gttcaacctt aatagtaacc catgcgctac
      241 ataaagtcaa agtgggaaaa agcccaccgg tcgccaccat ggtgagcaag ggcgaggagc
      301 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      361 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      421 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
      481 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      541 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      601 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      661 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
      721 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
      781 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
      841 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
      901 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
      961 ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct cgagctcaag
     1021 cttcgaattc tgcagtcgac ggtaccgcgg gcccggtgag gggctccctc tctggaaaag
     1081 tcagcctacc ttgtcatttt tcaacgatgc ctactttgcc acccagttac aacaccagtg
     1141 aatttctccg catcaaatgg tctaagattg aagtggacaa aaatggaaaa gatttgaaag
     1201 agactactgt ccttgtggcc caaaatggaa atatcaagat tggtcaggac tacaaaggga
     1261 gagtgtctgt gcccacacat cccgaggctg tgggcgatgc ctccctcact gtggtcaagc
     1321 tgctggcaag tgatgcgggt ctttaccgct gtgacgtcat gtacgggatt gaagacacac
     1381 aagacacggt gtcactgact gtggatgggg ttgtgtttca ctacagggcg gcaaccagca
     1441 ggtacacact gaattttgag gctgctcaga aggcttgttt ggacgttggg gcagtcatag
     1501 caactccaga gcagctcttt gctgcctatg aagatggatt tgagcagtgt gacgcaggct
     1561 ggctggctga tcagactgtc agatatccca tccgggctcc cagagtaggc tgttatggag
     1621 ataagatggg aaaggcagga gtcaggactt atggattccg ttctccccag gaaacttacg
     1681 atgtgtattg ttatgtggat catctggatg gtgatgtgtt ccacctcact gtccccagta
     1741 aattcacctt cgaggaggct gcaaaagagt gtgaaaacca ggctgccagg ctggcaacag
     1801 tgggggaact ccaggcggca tggaggaacg gctttgacca gtgcgattac gggtggctgt
     1861 cggatgccag cgtgcgccac cctgtgactg tggccagggc ccagtgtgga ggtggtctac
     1921 ttggggtgag aaccctgtat cgttttgaga accagacagg cttccctccc cctgatagca
     1981 gatttgatgc ctactgcttt aaacgtcgaa tgagtgattt gagtgtaatt ggtcatccaa
     2041 tagattcaga atctaaagaa gatgaacctt gtagtgaaga aacagatcca gtgcatgatc
     2101 taatggctga aattttacct gaattccctg acataattga aatagaccta taccacagtg
     2161 aagaaaatga agaagaagaa gaagagtgtg caaatgctac tgatgtgaca accaccccat
     2221 ctgtgcagta cataaatggg aagcatgtcg ttaccactgt gcccaaggac ccagaagctg
     2281 cagaagctag gcgtggccag tttgaaagtg ttgcaccttc tcagaatttc tcggacagct
     2341 ctgaaagtga tactcatcca tttgtaatag ccaaaacgga attgtctact gctgtgcaac
     2401 ctaatgaatc tacagaaaca actgagtctc ttgaagttac atggaagcct gagacttacc
     2461 ctgaaacatc agaacatttt tcaggtggtg agcctgatgt tttccccaca gtcccattcc
     2521 atgaggaatt tgaaagtgga acagccaaaa aaggggcaga atcagtcaca gagagagata
     2581 ctgaagttgg tcatcaggca catgaacata ctgaacctgt atctctgttt cctgaagagt
     2641 cttcaggaga gattgccatt gaccaagaat ctcagaaaat agcctttgca agggctacag
     2701 aagtaacatt tggtgaagag gtagaaaaaa gtacttctgt cacatacact cccactatag
     2761 ttccaagttc tgcatcagca tatgtttcag aggaagaagc agttacccta ataggaaatc
     2821 cttggccaga tgacctgttg tctaccaaag aaagctgggt agaagcaact cctagacaag
     2881 ttgtagagct ctcagggagt tcttcgattc caattacaga aggctctgga gaagcagaag
     2941 aagatgaaga tacaatgttc accatggtaa ctgatttatc acagagaaat actactgata
     3001 cactcattac tttagacact agcaggataa tcacagaaag cttttttgag gttcctgcaa
     3061 ccaccattta tccagtttct gaacaacctt ctgcaaaagt ggtgcctacc aagtttgtaa
     3121 gtgaaacaga cacttctgag tggatttcca gtaccactgt tgaggaaaag aaaaggaagg
     3181 aggaggaggg aactacaggt acggcttcta catttgaggt atattcatct acacagagat
     3241 cggatcaatt aattttaccc tttgaattag aaagtccaaa tgtagctaca tctagtgatt
     3301 caggtaccag gaaaagtttt atgtccttga caacaccaac acagtctgaa agggaaatga
     3361 cagattctac tcctgtcttt acagaaacaa atacattaga aaatttgggg gcacagacca
     3421 ctgagcacag cagtatccat caacctgggg ttcaggaagg gctgaccact ctcccacgta
     3481 gtcctgcctc tgtctttatg gagcagggct ctggagaagc tgctgccgac ccagaaacca
     3541 ccactgtttc ttcattttca ttaaacgtag agtatgcaat tcaagccgaa aaggaagtag
     3601 ctggcacttt gtctccgcat gtggaaacta cattctccac tgagccaaca ggactggttt
     3661 tgagtacagt aatggacaga gtagttgctg aaaatataac ccaaacatcc agggaaatag
     3721 tgatttcaga gcgattagga gaaccaaatt atggggcaga aataaggggc ttttccacag
     3781 gttttccttt ggaggaagat ttcagtggtg actttagaga atactcaaca gtgtctcatc
     3841 ccatagcaaa agaagaaacc gtaatgatgg aaggctctgg agatgcagca tttagggaca
     3901 cccagacttc accatctaca gtacctactt cagttcacat cagtcacata tctgactcag
     3961 aaggacccag tagcaccatg gtcagcactt cagccttccc ctgggaagag tttacatcct
     4021 cagctgaggg ctcaggtgag caactggtca cagtcagcag ctctgttgtt ccagtgcttc
     4081 ccagtgctgt gcaaaagttt tctggtacag cttcctccat tatcgacgaa ggattgggag
     4141 aagtgggtac tgtcaatgaa attgatagaa gatccaccat tttaccaaca gcagaagtgg
     4201 aaggtacgaa agctccagta gagaaggagg aagtaaaggt cagtggcaca gtttcaacaa
     4261 actttcccca aactatagag ccagccaaat tatggtctag gcaagaagtc aaccctgtaa
     4321 gacaagaaat tgaaagtgaa acaacatcag aggaacaaat tcaagaagaa aagtcatttg
     4381 aatcccctca aaactctcct gcaacagaac aaacaatctt tgattcacag acatttactg
     4441 aaactgaact caaaaccaca gattattctg tactaacaac aaagaaaact tacagtgatg
     4501 ataaagaaat gaaggaggaa gacacttctt tagttaacat gtctactcca gatccagatg
     4561 caaatggctt ggaatcttac acaactctcc ctgaagctac tgaaaagtca cattttttct
     4621 tagctactgc attagtaact gaatctatac cagctgaaca tgtagtcaca gattcaccaa
     4681 tcaaaaagga agaaagtaca aaacattttc cgaaaggcat gagaccaaca attcaagagt
     4741 cagatactga gctcttattc tctggactgg gatcaggaga agaagtttta cctactctac
     4801 caacagagtc agtgaatttt actgaagtgg aacaaatcaa taacacatta tatccccaca
     4861 cttctcaagt ggaaagtacc tcaagtgaca aaattgaaga ctttaacaga atggaaaatg
     4921 tggcaaaaga agttggacca ctcgtatctc aaacagacat ctttgaaggt agtgggtcag
     4981 taaccagcac aacattaata gaaattttaa gtgacactgg agcagaagga cccacggtgg
     5041 cacctctccc tttctccacg gacatcggac atcctcaaaa tcagactgtc aggtgggcag
     5101 aagaaatcca gactagtaga ccacaaacca taactgaaca agactctaac aagaattctt
     5161 caacagcaga aattaacgaa acaacaacct catctactga ttttctggct agagcttatg
     5221 gttttgaaat ggccaaagaa tttgttacat cagcaccaaa accatctgac ttgtattatg
     5281 aaccttctgg agaaggatct ggagaagtgg atattgttga ttcatttcac acttctgcaa
     5341 ctactcaggc aaccagacaa gaaagcagca ccacatttgt ttctgatggg tccctggaaa
     5401 aacatcctga ggtgccaagc gctaaagctg ttactgctga tggattccca acagtttcag
     5461 tgatgctgcc tcttcattca gagcagaaca aaagctcccc tgatccaact agcacactgt
     5521 caaatacagt gtcatatgag aggtccacag acggtagttt ccaagaccgt ttcagggaat
     5581 tcgaggattc caccttaaaa cctaacagaa aaaaacccac tgaaaatatt atcatagacc
     5641 tggacaaaga ggacaaggat ttaatattga caattacaga gagtaccatc cttgaaattc
     5701 tacctgagct gacatcggat aaaaatacta tcatagatat tgatcatact aaacctgtgt
     5761 atgaagacat tcttggaatg caaacagata tagatacaga ggtaccatca gaaccacatg
     5821 acagtaatga tgaaagtaat gatgacagca ctcaagttca agagatctat gaggcagctg
     5881 tcaacctttc tttaactgag gaaacatttg agggctctgc tgatgttctg gctagctaca
     5941 ctcaggcaac acatgatgaa tcaatgactt atgaagatag aagccaacta gatcacatgg
     6001 gctttcactt cacaactggg atccctgctc ctagcacaga aacagaatta gacgttttac
     6061 ttcccacggc aacatccctg ccaattcctc gtaagtctgc cacagttatt ccagagattg
     6121 aaggaataaa agctgaagca aaagccctgg atgacatgtt tgaatcaagc actttgtctg
     6181 atggtcaagc tattgcagac caaagtgaaa taataccaac attgggccaa tttgaaagga
     6241 ctcaggagga gtatgaagac aaaaaacatg ctggtccttc ttttcagcca gaattctctt
     6301 caggagctga ggaggcatta gtagaccata ctccctatct aagtattgct actacccacc
     6361 ttatggatca gagtgtaaca gaggtgcctg atgtgatgga aggatccaat cccccatatt
     6421 acactgatac aacattagca gtttcaacat ttgcgaagtt gtcttctcag acaccatcat
     6481 ctcccctcac tatctactca ggcagtgaag cctctggaca cacagagatc ccccagccca
     6541 gtgctctgcc aggaatagac gtcggctcat ctgtaatgtc cccacaggat tcttttaagg
     6601 aaattcatgt aaatattgaa gcaactttca aaccatcaag tgaggaatac cttcacataa
     6661 ctgagcctcc ctctttatct cctgacacaa aattagaacc ttcagaagat gatggtaaac
     6721 ctgagttatt agaagaaatg gaagcttctc ccacagaact tattgctgtg gaaggaactg
     6781 agattctcca agatttccaa aacaaaaccg atggtcaagt ttctggagaa gcaatcaaga
     6841 tgtttcccac cattaaaaca cctgaggctg gaactgttat tacaactgcc gatgaaattg
     6901 aattagaagg tgctacacag tggccacact ctacttctgc ttctgccacc tatggggtcg
     6961 aggcaggtgt ggtgccttgg ctaagtccac agacttctga gaggcccacg ctttcttctt
     7021 ctccagaaat aaaccctgaa actcaagcag ctttaatcag agggcaggat tccacgatag
     7081 cagcatcaga acagcaagtg gcagcgagaa ttcttgattc caatgatcag gcaacagtaa
     7141 accctgtgga atttaatact gaggttgcaa caccaccatt ttcccttctg gagacttcta
     7201 atgaaacaga tttcctgatt ggcattaatg aagagtcagt ggaaggcacg gcaatctatt
     7261 taccaggacc tgatcgctgc aaaatgaacc cgtgccttaa cggaggcacc tgttatccta
     7321 ctgaaacttc ctacgtatgc acctgtgtgc caggatacag cggagaccag tgtgaacttg
     7381 attttgatga atgtcactct aatccctgtc gtaatggagc cacttgtgtt gatggtttta
     7441 acacattcag gtgcctctgc cttccaagtt atgttggtgc actttgtgag caagataccg
     7501 agacatgtga ctatggctgg cacaaattcc aagggcagtg ctacaaatac tttgcccatc
     7561 gacgcacatg ggatgcagct gaacgggaat gccgtctgca gggtgcccat ctcacaagca
     7621 tcctgtctca cgaagaacaa atgtttgtta atcgtgtggg ccatgattat cagtggatag
     7681 gcctcaatga caagatgttt gagcatgact tccgttggac tgatggcagc acactgcaat
     7741 acgagaattg gagacccaac cagccagaca gcttcttttc tgctggagaa gactgtgttg
     7801 taatcatttg gcatgagaat ggccagtgga atgatgttcc ctgcaattac catctcacct
     7861 atacgtgcaa gaaaggaaca gttgcttgcg gccagccccc tgttgtagaa aatgccaaga
     7921 cctttggaaa gatgaaacct cgttatgaaa tcaactccct gattagatac cactgcaaag
     7981 atggtttcat tcaacgtcac cttccaacta tccggtgctt aggaaatgga agatgggcta
     8041 tacctaaaat tacctgcatg aacccatctg cataccaaag gacttattct atgaaatact
     8101 ttaaaaattc ctcatcagca aaggacaatt caataaatac atccaaacat gatcatcgtt
     8161 ggagccggag gtggcaggag tcgaggcgct gatccctaaa atggcgaaca tgtgttttca
     8221 tcatttcagc caaagtccta acttcctgtg cctttcctat cacctcgaca agcttgggct
     8281 gcaggtccgc cccccccccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     8341 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     8401 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     8461 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     8521 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     8581 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     8641 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     8701 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     8761 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     8821 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
     8881 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     8941 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     9001 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     9061 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     9121 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     9181 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     9241 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     9301 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     9361 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     9421 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     9481 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg
     9541 atgatctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     9601 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     9661 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     9721 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     9781 ggtgggctct atggcttctg aggcggaaag aacctgcagc aattcgccct atagtgagtc
     9841 gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     9901 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     9961 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaaac
    10021 gttaatattt tgttaaaatt cgcgttaaat atttgttaaa tcagctcatt ttttaaccaa
    10081 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
    10141 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
    10201 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt
    10261 ttgcggtcga ggtgccgtaa agctctaaat cggaacccta aagggagccc ccgatttaga
    10321 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
    10381 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
    10441 cttaatgcgc cgctacaggg cgcgtcgcct gatgcggtat tttctcctta cgcatctgtg
    10501 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    10561 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
    10621 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
    10681 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
    10741 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
    10801 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
    10861 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
    10921 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
    10981 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
    11041 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
    11101 gatgagcact tttaaagttc tgctatgtca tacactatta tcccgtattg acgccgggca
    11161 agagcaactc ggtcgccggg cgcggtattc tcagaatgac ttggttgagt actcaccagt
    11221 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
    11281 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
    11341 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    11401 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatgccaac
    11461 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    11521 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    11581 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    11641 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    11701 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    11761 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    11821 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    11881 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    11941 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    12001 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    12061 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
    12121 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    12181 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    12241 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    12301 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    12361 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    12421 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    12481 tcgatttttg tgatgctcgt caggggggcg gagcctatcg aaaaacgcca gcaacgcggc
    12541 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    12601 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
    12661 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
    12721 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
    12781 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    12841 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    12901 atttcacaca ggaaacagct atgaccatg
//
